Human Papillomavirus (HPV) DNA Detection with Genotyping, High Risk Types by PCR [A Test in Focus]

By Andy Tofilon • February 11, 2014

This new Mayo Clinic assay detects the 14 high-risk HPV types, and if present, provides specific genotype results for HPV-16 and/or HPV-18. Together, HPV types 16 and 18 cause more than 60 percent of cervical cancer in the United States. View a three-minute video interview with Dr. Matt Binnicker, which provides greater detail about this assay.

	HPV — Human Papillomavirus (HPV) DNA Detection with Genotyping, High Risk Types by PCR, ThinPrep View all test information details SHPV —Human Papillomavirus (HPV) DNA Detection with Genotyping, High Risk Types by PCR, SurePath View all test information details
	Detection of high risk (HR) genotypes associated with the development of cervical cancer An aid in triaging women with abnormal Pap smear results Individual genotyping of human papillomavirus (HPV)-16 and/or HPV-18, if present Results of HPV-16 and HPV-18 genotyping can be used as an aid in triaging women with positive HR-HPV but negative Pap smear results.
	HPV — Click here to view full specimen details. SHPV — Click here to view full specimen details.
	When should I order this test? This test may be ordered alongside cytology (i.e., co-testing) in women aged 30 to 65. In addition, this test may be used to assist in triaging women with abnormal (ASC-US) Pap smear results. Finally, the genotyping component of this test may be useful when co-testing reveals a positive result for high-risk HPV and normal cytology findings. Highlights: Up to 75% of women are exposed to human papilloma virus (HPV). Most infected women will clear the infection without any long-term health consequences. Persistent HPV infection is the principal cause of cervical cancer and its precursor lesions (cervical intraepithelial neoplasia). There are 14 HPV types (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68) that are classified as high-risk for the development of cervical cancer and its precursor lesions. HPV types 16 and 18 cause >60% of cervical cancer. Testing by PCR has become a standard method for determining the presence of a cervical HPV infection The Mayo Clinic assay detects the 14 high-risk HPV types, and if present, provides specific genotype results for HPV-16 and/or HPV-18.
	Real-Time Polymerase Chain Reaction (PCR) (PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)
	Day(s) and Time(s) Test Performed: Monday through Friday; Varies Analytic Time: 3 days