Mayo Clinic Laboratory and Pathology Research Roundup: July 31

By Kelley Luedke • July 31, 2017

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The Research Roundup provides an overview of the past week’s research from Mayo Medical Laboratories consultants, including featured abstracts and complete list of published studies and reviews.

Featured Abstract

Urinary Extracellular Vesicles of Podocyte Origin and Renal Injury in Preeclampsia

Renal histologic expression of the podocyte-specific protein, nephrin, but not podocin, is reduced in preeclamptic compared with normotensive pregnancies. Mayo Clinic researchers hypothesized that renal expression of podocyte-specific proteins would be reflected in urinary extracellular vesicles (EVs) of podocyte origin and accompanied by increased urinary soluble nephrin levels (nephrinuria) in preeclampsia. They further postulated that podocyte injury and attendant formation of EVs are related mechanistically to cell-free fetal hemoglobin (HbF) in maternal plasma. Compared with urine from women with normotensive pregnancies, urine from women with preeclamptic pregnancies contained a high ratio of podocin-positive to nephrin-positive urinary EVs (podocin+ EVs-to-nephrin+ EVs ratio) and increased nephrinuria, both of which correlated with proteinuria. Plasma levels of hemopexin, which were decreased in women with preeclampsia, negatively correlated with proteinuria, urinary podocin+ EVs-to-nephrin+ EVs ratio, and nephrinuria. Administration of HbF to pregnant rabbits increased the number of urinary EVs of podocyte origin. These findings provide evidence that urinary EVs are reflective of preeclampsia-related altered podocyte protein expression. Furthermore, renal injury in preeclampsia associated with an elevated urinary podocin+ EVs-to-nephrin+ EVs ratio and may be mediated by prolonged exposure to cellfree HbF. The study was published in the Journal of the American Society of Nephrology.

Published to PubMed This Week

• Flow Cytometry Based Monocyte Subset Analysis Accurately Distinguishes Chronic Myelomonocytic Leukemia from Myeloproliferative Neoplasms with Associated Monocytosis
  Blood Cancer Journal
• Upregulation of TET Activity with Ascorbic Acid Induces Epigenetic Modulation of Lymphoma Cells
  Blood Cancer Journal
  
• Composite Hemangioendothelioma with Neuroendocrine Marker Expression: An Aggressive Variant
  Modern Pathology
• Gastroblastoma Harbors a Recurrent Somatic MALAT1-GLI1 Fusion Gene
  Modern Pathology
• Assessment of Renal Response with Urinary Exosomes in Patients with AL Amyloidosis: A Proof of Concept
  American Journal of Hematology
• Targeted Next-Generation Sequencing and Identification of Risk Factors in World Health Organization Defined Atypical Chronic Myeloid Leukemia
  American Journal of Hematology
• EGFR as a Prognostic Biomarker and Therapeutic Target in Ovarian Cancer: Evaluation of Patient Cohort and Literature Review
  Genes Cancer
• Unilateral Vestibular Schwannoma and Meningiomas in a Patient with PIK3CA-Related Segmental Overgrowth: Co-occurrence of Mosaicism for Two Rare Disorders
  Clinical Genetics
• Breast Implant Capsule-Associated Squamous Cell Carcinoma: A Report of Two Cases
  Human Pathology
• Comprehensive Immune Profiling Reveals Substantial Immune System Alterations in a Subset of Patients with Amyotrophic Lateral Sclerosis
  PLoS One
• Assessing a Single Targeted Next Generation Sequencing for Human Leukocyte Antigen Typing Protocol for Interoperability, as Performed by Users with Variable Experience
  Human Immunology
• Cost and Quality Issues in Establishing Hematopoietic Cell Transplant Program in Developing Countries
  Hematology/Oncology and Stem Cell Therapy
• Molecular Malfeasance Mediating Myeloid Malignancies: The Genetics of Acute Myeloid Leukemia
  Methods in Molecular Biology
• Opsoclonus-Myoclonus Syndrome during Rituximab Treatment for Autoimmune Autonomic Ganglionopathy
  Neurology: Neuroimmunology & Neuroinflammation

Kelley Luedke

Kelley Luedke is a Marketing Channel Manager at Mayo Clinic Laboratories. She is the principle editor and writer of Insights and leads social media and direct marketing strategy. Kelley has worked at Mayo Clinic since 2013. Outside of work, you can find Kelley running, traveling, playing with her kitty, and exploring new foods.