Mayo Clinic Laboratory and Pathology Research Roundup: Oct. 22

By Kelley Luedke • October 22, 2018

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The Research Roundup provides an overview of the past week’s research from Mayo Medical Laboratories consultants, including featured abstracts and complete list of published studies and reviews.

Featured Abstract

Neoantigenic Potential of Complex Chromosomal Rearrangements in Mesothelioma

Malignant pleural mesothelioma is a disease primarily associated with exposure to the carcinogen asbestos. Whereas other carcinogen-related tumors are associated with a high tumor mutation burden, mesothelioma is not. Mayo Clinic researchers sought to resolve this discrepancy. They used mate-pair, RNA, and T cell receptor sequencing along with in silico predictions and immunologic assays to understand how structural variants of chromosomes affect the transcriptome. Researchers observed that inter- or intra-chromosomal rearrangements were present in every specimen and were frequently in a pattern of chromoanagenesis such as chromoplexy or chromothripsis. Transcription of rearrangement-related junctions was predicted to result in many potential neoantigens, some of which were proven to bind patient-specific MHC molecules and to expand intratumoral T cell clones. T cells responsive to these predicted neoantigens were also present in a patient's circulating T cell repertoire. Analysis of genomic array data from the mesothelioma cohort in The Cancer Genome Atlas suggested that multiple chromothriptic-like events negatively impact survival. The findings represent the discovery of potential neoantigen expression driven by structural chromosomal rearrangements. These results may have implications for the development of novel immunotherapeutic strategies and the selection of patients to receive immunotherapies. The study was published in the Journal of Thoracic Oncology,

Published to PubMed This Week

• • • • • Determinants of Long-Term Outcome in Type 1 Calreticulin-Mutated Myelofibrosis
          Leukemia
        • The Fentanyl Epidemic and Evolution of Fentanyl Analogs in the United States and the European Union
          Clinical Chemistry
        • Loss of TNFAIP3 Enhances MYD88 L265P-Driven Signaling in Non-Hodgkin Lymphoma
          Blood Cancer Journal
        • Ex-PRESS Shunt Extrusion upon Globe Palpation
          Ophthalmology
        • CKD Due to a Novel Mitochondrial DNA Mutation: A Case Report
          American Journal of Kidney Diseases
        • Aberrant Receptor Tyrosine Kinase Signaling in Lipofibromatosis: A Clinicopathological and Molecular Genetic Study of 20 cases
          Modern Pathology
        • Recurrent Genomic Alterations in Soft Tissue Perineuriomas
          American Journal of Surgical Pathology
        • Adamantinoma of Bone: Long-Term Follow-up of 46 Consecutive Patients
          Journal of Surgical Oncology
        • A Rapid and Reliable Chromosome Analysis Method for Products of Conception Using Interphase Nuclei
          Molecular Genetics & Genomic Medicine
        • Red Cell Exchange for a Case of Babesiosis
          Journal of Clinical Apheresis
        • The Characteristics of All-Terrain Vehicle (ATV)-Related Deaths: A Forensic Autopsy Data-Based Study
          Forensic Science, Medicine, and Pathology
        • Implementation of a Pharmacogenomics Education Program for Pharmacists
          American Journal of Health-System Pharmacy
        • Comparative Evaluation of cDNA Library Construction Approaches for RNA-Seq Analysis from Low RNA-Content Human Specimens
          Journal of Microbiological Methods
        • Adult-Onset Nonparalytic, Small-Angle Hypertropia
          JAAPOS
        • EUS Fine-Needle Pancreatic Core Biopsy Can Determine Eligibility for Tumor-Agnostic Immunotherapy
          Endoscopy International Open
        • A Case of IgG4-Related Aortitis and Pericarditis: Diagnostic Challenges and Natural History
          American Journal of Case Reports

Kelley Luedke

Kelley Luedke is a Marketing Channel Manager at Mayo Clinic Laboratories. She is the principle editor and writer of Insights and leads social media and direct marketing strategy. Kelley has worked at Mayo Clinic since 2013. Outside of work, you can find Kelley running, traveling, playing with her kitty, and exploring new foods.