Mayo Clinic Laboratory and Pathology Research Roundup: June 18

By Kelley Luedke • June 18, 2018

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The Research Roundup provides an overview of the past week’s research from Mayo Medical Laboratories consultants, including featured abstracts and complete list of published studies and reviews.

Featured Abstract

Highly Discriminant Methylated DNA Markers for the Non-Endoscopic Detection of Barrett's Esophagus

Minimally invasive methods have been described to detect Barrett's esophagus (BE), but are limited by subjectivity and suboptimal accuracy. Mayo Clinic researchers identified methylated DNA markers (MDMs) for BE in tissue and assessed their accuracy on whole esophagus brushings and capsule sponge samples. Unbiased whole methylome sequencing was performed on DNA from BE and normal squamous esophagus (SE) tissue.

Discriminant MDM candidates were validated on an independent patient cohort by quantitative methylation specific PCR (qMSP). Selected MDMs were further evaluated on whole esophageal brushings. 35 previously sequenced esophageal adenocarcinoma (EAC) MDMs were also evaluated. 20 BE cases and 20 controls were randomized to swallow capsules sponges followed endoscopy. DNA yield, tolerability, and mucosal injury were compared. Best MDM assays were performed on this cohort. Researchers identified MDMs discriminate BE with high accuracy. BE detection appears safe and feasible with a capsule sponge. Corroboration in larger studies is warranted. The study was published in American Journal of Gastroenterology.

Published to PubMed This Week

• • • • • Comprehensive Annotation of BRCA1 and BRCA2 Missense Variants by Functionally Validated Sequence-Based Computational Prediction Models
          Genetic Medicine
        • Validation of the WHO-Defined 20% Circulating Blasts Threshold for Diagnosis of Leukemic Transformation in Primary Myelofibrosis
          Blood Cancer Journal
        • DNA Mismatch Repair Protein Deficient Non-Neoplastic Colonic Crypts: A Novel Indicator of Lynch Syndrome
          Modern Pathology
        • In Vitro Activity of Oritavancin Against Biofilms of Staphylococci Isolated from Prosthetic Joint Infection
          Diagnostic Microbiology and Infectious Disease
        • Validation of Osteogenic Properties of Cytochalasin D by High-Resolution RNA-Sequencing in Mesenchymal Stem Cells Derived from Bone Marrow and Adipose Tissues
          Stem Cells and Development
        • Subconjunctival Exposure to Carbopol Causes Chronic Histiocytic Inflammatory Response in Rabbits
          Translational Vision Science & Technology
        • Current Approaches to Cancer Genetic Counseling Services for Spanish-Speaking Patients
          Journal of Immigrant and Minority Health

Kelley Luedke

Kelley Luedke is a Marketing Channel Manager at Mayo Clinic Laboratories. She is the principle editor and writer of Insights and leads social media and direct marketing strategy. Kelley has worked at Mayo Clinic since 2013. Outside of work, you can find Kelley running, traveling, playing with her kitty, and exploring new foods.