The research roundup provides an overview of the past week’s research from Mayo Clinic Laboratories consultants, including featured abstracts and a complete list of published studies and reviews.
Sustained Perfusion of Revascularized Bioengineered Livers Heterotopically Transplanted into Immunosuppressed Pigs.
Implanted bioengineered livers have not exceeded three days of continuous perfusion. Here we show that decellularized whole porcine livers revascularized with human umbilical vein endothelial cells and implanted heterotopically into immunosuppressed pigs whose spleens had been removed can sustain perfusion for up to 15 days. We identified peak glucose consumption rate as a main predictor of the patency of the revascularized bioengineered livers (rBELs). Heterotopic implantation of rBELs into pigs in the absence of anticoagulation therapy led to sustained perfusion for three days, followed by a pronounced immune responses directed against the human endothelial cells. A 10 day steroid-based immunosuppression protocol and a splenectomy at the time of rBEL implantation reduced the immune responses and resulted in continuous perfusion of the rBELs for over two weeks. We also show that the human endothelial cells in the perfused rBELs colonize the liver sinusoids and express sinusoidal endothelial markers similar to those in normal liver tissue. Revascularized liver scaffolds that can maintain blood perfusion at physiological pressures might eventually help to overcome the chronic shortage of transplantable human livers. Via National Biomedical Engineering.
Published to PubMed This Week
- A Randomized Double-Blind Phase II Study of The Seneca Valley Virus (NTX-010) vs Placebo for Patients with Extensive Stage SCLC (ES-SCLC) Who Were Stable or Responding After At Least 4 Cycles of Platinum-Based Chemotherapy: NCCTG (Alliance) N0923 Study.
Journal of Thoracic Oncology
- SIRPα Expression Delineates Subsets of Intratumoral Monocyte/Macrophages with Different Functional and Prognostic Impact in Follicular Lymphoma.
Blood Cancer Journal
- Whole Exome Sequencing Among 26 Patients With Indeterminate Acute Liver Failure: A Pilot Study.
Clinical and Translational Gastroenterology
- Multicenter Study Demonstrates Standardization Requirements for Mold Identification by MALDI-TOF MS.
Frontiers in Microbiology
- Matrix-Delivered Autologous Mesenchymal Stem Cell Therapy for Refractory Rectovaginal Crohn's Fistulas.
Inflammatory Bowel Disease
- Role of Lymphadenectomy in Endometrial Cancer with Nonbulky Lymph Node Metastasis: Comparison of Comprehensive Surgical Staging and Sentinel Lymph Node Algorithm.
- Histology Findings In Vitreorentinal Lymphoma: Learning From Enucleation Specimens.
- Adverse Renal Response to Decongestion in the Obese Phenotype of Heart Failure with Preserved Ejection Fraction.
Journal of Cardiac Failure
- PD-1/PD-L1 Expression in Extramedullary Lesions of Acute Myeloid Leukemia.
Leukemia and Lymphoma
- Clinical Outcome of Patients Diagnosed with Myelodysplastic Syndrome-Unclassifiable (MDS-U): Single Center Experience.
Leukemia and Lymphoma
- Prophylactic Intravenous Access: Is It Necessary for Renal Transplant Biopsies?
Current Problems in Diagnostic Radiology