Mayo Clinic laboratory and pathology research roundup: November 23
The research roundup provides an overview of the past week’s research from Mayo Clinic Laboratories consultants, including featured abstracts and a complete list of published studies and reviews.
Regulation of Direct Adipose Tissue Free Fatty Acid Storage During Mixed Meal Ingestion and High Free Fatty Acid Concentration Conditions.
Direct free fatty acid (FFA) storage into adipocytes relates to body fat distribution. Adipose tissue CD36, acyl-CoA synthetase (ACS), and diacylglycerol acetyl-transferase (DGAT) may account for some of the between-depot and inter-individual variability in FFA storage. These studies were to test whether CD36, ACS or DGAT might be important for direct palmitate storage under meal-ingestion or high FFA conditions. We measured upper (UBSQ) and lower body subcutaneous (LBSQ) adipose tissue FFA storage rates by infusing palmitate tracers intravenously and performing adipose biopsies under hypoinsulinemic (high FFA) and mixed meal conditions. We recruited 5 postmenopausal women, physically active males (5) and females (5), and sedentary males (5) and females (5). We found: 1) the ratio of UBSQ to LBSQ DGAT activity predicted the ratio of palmitate storage (adjusted R = 0.25, F = 8.0, P = 0.01, 95% CI (0.07, 0.48)) under high FFA conditions; 2) the ratio of UBSQ to LBSQ ACS activity predicted the ratio of palmitate storage under meal conditions (adjusted R = 0.18, F = 6.3, P = 0.02, 95% CI (0.12, 1.28); 3) LBSQ direct palmitate storage rates were significantly less in physically active than sedentary adults; 3); 4) adipose tissue CD36 protein content, ACS or DGAT activities did not independently predict palmitate storage rates. We conclude that physically active adults have lesser fatty acid cycling back into adipose tissue and that adipose ACS and DGAT may affect competition between UBSQ and LBSQ adipose for direct palmitate storage. Via American Journal of Physicology. Endocrinology and Metabolism.
Published to PubMed This Week
- Plasmid Acquisition Alters Vancomycin Susceptibility in Clostridioides Difficile.
- Use of Autologous Stem Cells Cryopreserved for Over 15 Years in Stem Cell Transplantation for Multiple Myeloma.
Bone Marrow Transplant
- Prevalence and Phenotypic Correlations of Calmodulinopathy-Causative CALM1-3 Variants Detected in a Multi-Center Molecular Autopsy Cohort of Sudden Unexplained Death Victims.
Circulation. Genomics and Precision Medicine
- Low Dose CT Image and Projection Dataset.
- COVID-19-Associated Non-Occlusive Fibrin Microthrombi in the Heart.
- Updated Ferritin Reference Intervals for the Roche Elecsys® Immunoassay.
- Evaluation of Sporadic Bovine Alkaline Phosphatase Interference in the Beckman Access Unconjugated Estriol (uE3) Assay Affecting Maternal Serum Screening Results.
- Clinical Laboratory Validation of the MCL35 Assay for Molecular Risk Stratification of Mantle Cell Lymphoma.
Journal of Hematopathology
- Rifampin, Rifapentine, and Rifabutin are Active Against Intracellular Periprosthetic Joint Infection-Associated Staphylococcus epidermidis.
Antimicrobial Agents and Chemotherapy