Melanoma tumors vary greatly, complicating decisions about optimal patient care. In this test specific episode of the "Answers From the Lab" podcast, Ruifeng (Ray) Guo, M.D., Ph.D., explains how Mayo Clinic Laboratories' comprehensive MayoComplete Melanoma Panel better informs prognosis and treatment.

"This updated version of the panel adds 12 genes associated with melanoma and uses whole exome sequencing. It will be very helpful for better classification of melanoma, which is critical for clinical treatment guidance," Dr. Guo says.

The assay can be applied to a wide variety of cases, including unusual tumors that aren't yet diagnosed as melanoma and complex melanomas whose molecular signatures can guide prognosis and treatment. "This new panel is for a broader population of patients and provides more informative molecular testing for melanoma," Dr. Guo says.

Microsatellite instability (MSI) status — an emerging predictive and prognostic biomarker for immunotherapy response in cancer — is included in the new assay. "The addition of MSI will definitely provide more clinical and molecular insight for the classification of melanoma, with the potential for MSI-targeted therapy in the future," Dr. Guo says.

Listen to learn more about Mayo Clinic Laboratories' updated MayoComplete Melanoma Panel.

Note: Podcasts will not playback on Internet Explorer. Please use an alternative web browser, or listen from your mobile device on a preferred listening app.

Testing

MCMLN | MayoComplete Melanoma Panel

Useful information

• Diagnosis and management of patients with melanoma
• Assessment of microsatellite instability

Specimen requirements

This assay requires at least 20% tumor nuclei

• Preferred amount of tumor area with sufficient percent tumor nuclei: tissue 216mm(2)
• Minimum amount of tumor area: tissue 36mm(2)
• These amounts are cumulative over up to 10 unstained slides and must have adequate percent tumor nuclei
• Tissue fixation: 10% neutral buffered formalin, not decalcified

For specimen preparation guidance, see Tissue Requirement for Solid Tumor Next-Generation Sequencing. In this document, the sizes are given as 4mm x 4mm x 10 slides as preferred: approximate/equivalent to 144 mm(2) and the minimum as 3mm x 1mm x 10 slides: approximate/equivalent to 36mm(2).

Preferred:

• Specimen Type: Tissue block
• Collection Instructions: Submit a formalin-fixed, paraffin-embedded tissue block with acceptable amount of tumor tissue

Acceptable:

• Specimen Type: Tissue slide
• Slides: 1 Stained and 10 unstained
• Collection Instructions: Submit 1 slide stained with hematoxylin and eosin and 10 unstained, nonbaked slides wit 5-micron thick sections of the tumor tissue

Note: The total amount of required tumor nuclei can be obtained by scraping up to 10 slides from the same block

Additional Information: Unused unstained slides will not be returned

• Specimen Type: Cytology slide (direct smears or ThinPrep)
• Slides: 1 to 3 Slides

Collection Instructions: Submit 1 to 3 slides stained and cover slipped with a preferred total of 5000 nucleated cells, or a minimum of at least 3000 nucleated cells

Note: Glass coverslips are preferred; plastic coverslips are acceptable but will result in longer turnaround times

Additional Information: Cytology slides will not be returned

Performance information

Analytic time: 12 days

Days performed: Monday through Friday

The latest

Assay identifies patients eligible for new ovarian cancer drug: Maryam Shahi, M.D.

April 23, 2024

Maryam Shahi, M.D., explains how Mayo Clinic Laboratories' unique biomarker test (TEST ID: AFOLR) determines which patients would likely benefit from a new treatment for recurrent epithelial ovarian cancer. Disease recurrence is common, and about one-third of patients respond to the new medication.

Assay monitors response to monoclonal antibody therapy: Maria Alice Willrich, Ph.D.

April 16, 2024

Maria Alice Willrich, Ph.D., explains how Mayo Clinic Laboratories' new assay provides therapeutic drug monitoring of risankizumab, or RISA. Test results help guide care for patients with plaque psoriasis, psoriatic arthritis, and Crohn's disease.

Noninvasive plasma biomarker testing provides faster answers: Alicia Algeciras-Schimnich, Ph.D. 

April 9, 2024

Alicia Algeciras-Schimnich, Ph.D., discusses Mayo Clinic Laboratories’ new noninvasive plasma biomarker assay for Alzheimer’s disease, an accessible, highly accurate testing option for individuals age 50 and above who are experiencing mild cognitive impairment or early dementia. Positive test results can confirm amyloid beta pathology and facilitate access to disease-modifying therapies.

View MoreView Less