Updated assay guides diagnosis of autoimmune liver disease

Answers from the Lab

Diagnosis of autoimmune liver disease can be challenging. In this test-specific episode of the "Answers From the Lab" podcast, Anne Tebo, Ph.D., explains how Mayo Clinic Laboratories' updated ALDG2 assay helps with the evaluation of patients with suspected autoimmune hepatitis, primary biliary cholangitis (PBC), and liver disease of unknown etiology.

"The disease spectrum is broad, and disease boundaries may be blurred by overlapping features," Dr. Tebo says. "The panel should ideally be requested following observation of abnormal liver enzymes, as well as for the elimination of common causes of liver disease such as metabolic, genetic, viral, drug, or alcohol-induced hepatitis."

The assay includes tests for two types of antibodies associated with autoimmune hepatitis and one type that is highly suggestive of PBC. Test results should be interpreted in the context of clinical assessment.

"Detection of these antibodies in asymptomatic patients may suggest risk for future disease development, which would require close monitoring for disease onset," Dr. Tebo says.

Listen to learn more about Mayo Clinic Laboratories' ALDG2 assay.

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Useful information

Evaluating patients with suspected autoimmune liver disease, specifically autoimmune hepatitis or primary biliary cholangitis.

Evaluating patients with liver disease of unknown etiology.

Specimen requirements

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)


Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.

Performance information

  • Analytic time: 3 - 4 days
  • Day(s) and time(s) performed: Monday through Saturday

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Barbara J. Toman

Barbara J. Toman is a Senior Communications Specialist at Mayo Clinic Laboratories. She is also the science writer for Mayo’s Neurosciences Update newsletter, which helps referring physicians to stay informed about Mayo’s treatment and research. Barbara has worked at Mayo Clinic since 2007. She enjoys international travel and cooking.