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Mayo Clinic Laboratories > Infectious diseases > Sexually transmitted infection > Human immunodeficiency virus (HIV)

Human immunodeficiency virus (HIV) TESting

Clarify infection status

For individuals who have been exposed to human immunodeficiency virus (HIV), testing is imperative to not only access treatments that can inhibit the infection’s progression to acquired immunodeficiency syndrome (AIDS), but also to prevent spreading the infection to sexual partners.

We offer a full menu of HIV-1 and HIV-2 testing, including screening, diagnostic, and confirmatory testing. Guided by an algorithmic approach, our testing aligns with recommendations put forth by the U.S. Centers for Disease Control and Prevention (CDC).

 ~1 in 8

 ~1 in 8 people in the U.S. who have HIV do not know they have it1

HIV test menu

Screening Screening
Diagnostic testing Diagnostic testing
Confirmatory testing Confirmatory testing

Screening

For nonsymptomatic, nonpregnant people who have been exposed to HIV infection, screening for HIV-1/-2 antigens and antibodies can detect HIV infection.

Key testing

Diagnostic testing

For symptomatic individuals or in cases when initial HIV screening is reactive, or positive, diagnostic testing offers further proof of an HIV infection.

Key testing

Confirmatory testing

For cases where initial screening performed at outside labs returns a positive result, confirmation and differentiation of HIV-1 and HIV-2 antibodies is available.

Key testing

Download HIV Testing Algorithm (Fourth-Generation Screening Assay),
Including Follow-up of Reactive Rapid Serologic Test Results
References
  1. Who Should Get Tested for HIV? U.S. Department of Health & Human Services. https://www.hiv.gov/hiv-basics/hiv-testing/learn-about-hiv-testing/who-should-get-tested. Published December 15, 2021. Accessed February 6, 2026.