Invasive fungal infection
Advanced analysis to clarify a difficult diagnosis
For immunosuppressed patients with nonspecific symptoms due to invasive fungal infections (IFI), diagnosis remains a challenge. Limitations of traditional laboratory testing often result in delayed diagnosis, which is associated with high mortality rates.
Mayo Clinic Laboratories offers rapid in vitro diagnostic screening tests for IFI caused by various fungi, including Aspergillus species, Fusarium species, and Candida species, among others.
Invasive fungal infections Test menu
Serologic testing
Fungal biomarker detection, used in conjunction with conventional testing methods, can help to make a presumptive diagnosis of fungal infection prior to symptom progression.
- Elevated serum levels of β-D-glucan (BDG) are associated with the presence of fungal infections.
- Detection of Aspergillus galactomannan antigen in serum and bronchoalveolar lavage specimens is associated with invasive aspergillosis.
- Histoplasma and Blastomyces antigen detection in serum and urine is frequently used to rapidly identify infections with these fungal pathogens.
Key testing
Advantages
- Detects circulating BDG in serum.
- Aids in identifying deep-seated fungal infections of fungemia in patients with symptoms of, or with a medical condition predisposing them to, an invasive fungal infection.
- Note: When positive, repeat testing on a new sample (collected in 3–4 days) is recommended, as serially positive samples are associated with a higher diagnostic odds ratio for invasive fungal infection compared to a single positive result.
Recommended test pairings
Recommended laboratory test pairings are frequently used in conjunction with BDG testing.
Fungal culture
Direct examination
Polymerase chain reaction
- PNRP | Pneumocystis jiroveci, Molecular Detection, PCR, Varies
- HBRP | Histoplasma capsulatum/Blastomyces species, Molecular Detection, PCR, Varies
- CIMRP | Coccidioides immitis/posadasii, Molecular Detection, PCR, Varies
- CIMT | Coccidioides immitis/posadasii, Molecular Detection, PCR, Paraffin, Tissue
Additional testing
Aspergillus
- ASPBA | Aspergillus Antigen, Bronchoalveolar Lavage
- ASPAG | Aspergillus (Galactomannan) Antigen, Serum
Blastomyces
- BLAST | Blastomyces Antibody, Enzyme Immunoassay, Serum
- SBL | Blastomyces Antibody Immunodiffusion, Serum
- CBL | Blastomyces Antibody Immunodiffusion, Spinal Fluid
- HBRP | Histoplasma capsulatum/Blastomyces species, Molecular Detection, PCR, Varies
- HIBAG | Histoplasma and Blastomyces Antigen, Enzyme Immunoassay, Serum
- UHBAG | Histoplasma/Blastomyces Antigen, Enzyme Immunoassay, Urine
Coccidioides
- RSCOC | Coccidioides Antibody Reflex, Complement Fixation and Immunodiffusion, Serum
- SCOC | Coccidioides Antibody, Complement Fixation and Immunodiffusion, Serum
- CCOC | Coccidioides Antibody, Complement Fixation and Immunodiffusion, Spinal Fluid
- COXIS | Coccidioides Antibody Screen with Reflex, Serum
- CIMT | Coccidioides immitis/posadasii, Molecular Detection, PCR, Paraffin, Tissue
- CIMRP | Coccidioides immitis/posadasii, Molecular Detection, PCR, Varies
Cryptococcus
- PLFA | Cryptococcus Antigen Screen, Lateral Flow Assay, Pleural Fluid
- PLFAT | Cryptococcus Antigen Titer, Lateral Flow Assay, Pleural Fluid
- SLFAT | Cryptococcus Antigen Titer, Lateral Flow Assay, Serum
- CLFAT | Cryptococcus Antigen Titer, Lateral Flow Assay, Spinal Fluid
- ULFA | Cryptococcus Antigen Screen, Lateral Flow Assay, Random, Urine
- SLFA | Cryptococcus Antigen Screen with Titer, Serum
- CLFA | Cryptococcus Antigen Screen with Titer, Spinal Fluid
- LFACX | Cryptococcus Antigen with Reflex, Spinal Fluid
Histoplasma
- HBRP | Histoplasma capsulatum/Blastomyces species, Molecular Detection, PCR, Varies
- HIBC | Histoplasma/Blastomyces Panel, Spinal Fluid
- HIBAG | Histoplasma/Blastomyces Antigen, Enzyme Immunoassay, Serum
- HICSF | Histoplasma Antibody Complement Fixation and Immunodiffusion, Spinal Fluid
- HISER | Histoplasma Antibody Complement Fixation and Immunodiffusion, Serum
- UHBAG | Histoplasma/Blastomyces Antigen, Enzyme Immunoassay, Urine