The Mayo Clinic Special Coagulation Laboratory has been at the forefront of patient-centered and value-based diagnostic laboratory testing, research, and patient management for nearly 90 years. Led by a multidisciplinary team of physicians, the laboratory offers a comprehensive repertoire of coagulation tests covering a wide spectrum of bleeding and thrombotic disorders.
Platelet transmission electron microscopy (PTEM) has been an essential tool for laboratory diagnosis of various hereditary platelet disorders since it was first used to visualize fibrin-platelet clot formation in 1955. PTEM employs two main methods to visualize platelet ultrastructure: whole mount (WM) TEM and thin section (TS) TEM. WM-TEM is considered the gold-standard test for diagnosing dense granule deficiencies in Hermansky-Pudlak syndrome, alpha-delta platelet storage pool deficiency, Paris-Trousseus-Jacobsen syndrome, Wiskott-Aldrich syndrome, TAR (thrombocytopenia, absent radii) syndrome, Chediak-Higashi syndrome, and more.
Platelet flow cytometric analysis is the preferred method to assess hereditary platelet disorders due to quantitative surface glycoprotein (GP) deficiencies. GP expression levels can be measured by using fluorescent-conjugated GP-specific antibodies, and their fluorescent intensities can be compared to normal ranges of various glycoproteins.
Thrombotic microangiopathy is a group of disorders that is characterized by thrombocytopenia and microangiopathic hemolytic anemia (intravascular hemolysis and presence of peripheral blood schistocytes), neurological symptoms, fever, and renal dysfunction. Ischemic organ injury can occur to the brain, kidneys, heart, pancreas, liver, and lungs.
Complement testing has acquired considerable clinical popularity in recent times. As the medical industry gains a better understanding of its role in infectious, autoimmune, and inflammatory diseases, complement testing is progressively being recognized as a potential target for therapeutics. An overview of the complement system, pre-analytical challenges, and modern laboratory testing will be presented in this webinar.